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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in many tumors, which results in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was located to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a vital position in selling mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have already been described. Two further splice variants have been noted but thorough nucleotide sequence information just isn't nevertheless accessible.
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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide usually includes the epitope recognized by the antibody.
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This tutorial outlines even further achievable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This guidebook outlines even more doable optimization read more of mobile and immunoassay parameters to guarantee the very best effects are...
Antibodies sure to the blocking peptide now not bind towards the epitope around the focus on protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is specific.
Target Details Cyclin E belongs for the really conserved cyclin family, whose associates are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates for the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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